![]() Quantitative: Novel RenSP luciferase fusion technology allows you to measure promoter activity in response to NF-kB pathway activation with industry-leading sensitivity and dynamic range.The Power of the LightSwitch Luciferase Assay System Read more about this technology.Ĭlick on the image below for a larger view. SwitchGear also offers 3′UTR reporters for studying transcriptional stability, translational repression, and miRNA function. Screen for promoter activation or develop activity profiles across the NF-kB signaling pathway for many compounds or conditions in parallel.Measure the effect of sequence variants and mutagenesis on promoter function.Confirm data from ChIP-chip or ChIP-sequencing experiments.Quantify the functional consequences of transcription factor binding – while many other technologies give a qualitative view of transcription factor level, our promoter reporter assays quantify the effects of transcription factor binding.Understand the mechanisms by which NF-kB regulated genes are induced or repressed.The SwitchGear collection of NF-kB -relevant promoter reporters allows you to: In addition, SwitchGear offers UTR reporter assays that measure transcript stability, translational repression, miRNA function. SwitchGear Genomics offers a comprehensive panel of transfection-ready, cell-based promoter reporter assays to allow you to accurately quantify transcriptional activation and repression in response to NF-kB pathway activation. Understanding the NF-kb pathway may aid in identifying preventive and therapeutic measures for diseases, such as chronic inflammation and cancer. Improper regulation of NF-κB has been associated with various cancers, autoimmune diseases, inflammatory disorders, and viral infection. NF-kB family members - NF-kB1 (p50), NF-kB2 (p52), RelA (p65), RelB, and c-Rel share a highly conserved domain responsible for DNA binding to NF-kB-elements. Various signals such as intracellular stress, cytokines, UV irradiation, free radicals, and infection induce gene expression through the NF-kB family. NF-kB is a ubiquitous transcription factor that regulates the genes that mediate cell cycle, apoptosis, intercellular communication, immune response and development, memory formation, and tumorigenesis.
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